Jewawut (Setaria italica L.) adalah tanaman sereal anggota keluara rumput Poaceae. Akar jewawut diklasifikasikan sebagai akar serabut dan terdiri dari akar prime dan skunder yang saat ini belum ada penelitian pengenai baktri rhizosfer penambat nitrogen pada perakaran tanaman jewawut. Penelitian ini bertujuan untuk mengetahui isolasi dan uji kemampuan bakteri penambat nitrogen dari rhozosfer perakaran tanaman jewawut untuk menghasilkan sumber belajar biologi dalam bentuk booklet. Pendekatan yang digunakan pada penelitian kuantitatif, sampel pada penelitian ini adalah rhozosfer perakaran dari tanaman jewawut. Data penelitian dianalisis secara deskriptif kuantitatif yaitu meliputi karakteristik makroskopis dan mikroskopis yang meliputi uji penambat nitroge, uji IAA dan Giberelin GA3 serta identifikasi bakteri berbasis 16S rRNA dari masing-masing bakteri yang berhasil diisolasi dari rhozosfer perakaran tanaman jewawut. Hasil penelitian menunjukkan bahwa isolat yang dapat menembat nitrogen yaitu jewawut muda pinggir rhizosfer (JMPR), pewarnaan Gram 5 isolat bergram negatif dan 5 isolat lainnya bergram positif dengan bentuk didominasi coccus. Uji penambat nitrogen 5 isolat dapat menembat nitrogen dilihat adannya cincin yang terbentuk dengan kode isolat JMPR19, JMPR22,JMPR28,JMPR29, dan JMPR31. Sebanyak 3 isolat dapat menghasilkan IAA dan Giberelin GA3 dan 2 isolat berhasil dianalisi BLAST yang menunjukkan tingkat kemiripannya pada species Bacillus Toyonensis dan Bacillus Cereus. Hasil penelitian ini kemudian diimplementasikan ke dalam bentuk booklet sebagai sumber belajar biologi dengan materi bakteri.
Millet (Setaria italica L.) is a cereal plant belonging to the grass family Poaceae. Millet roots are classified as fibrous roots and consist of prime and secondary roots. Currently there has been no research on nitrogen-fixing rhizosphere bacteria in the roots of millet plants. This research aims to isolate and test the ability of nitrogen-fixing bacteria from the rhozosphere of millet plant roots to produce biology learning resources in the form of booklets. The approach used in quantitative research, the sample in this study was the root rhozosphere of millet plants. The research data was analyzed descriptively quantitatively, which included macroscopic and microscopic characteristics which included the nitrogen fixing test, IAA and Gibberellin GA3 tests as well as identification of bacteria
based on 16S rRNA from each bacteria that was isolated from the rhozosphere of millet plant roots. The results of the research showed that the isolates that could fix nitrogen were young barley at the edge of the rhizosphere (JMPR), the Gram staining of 5 isolates was gram negative and the other 5 isolates were gram positive with a form dominated by coccus. Nitrogen fixing test: 5 isolates were
able to fix nitrogen by looking at the rings formed with the isolate codes JMPR19, JMPR22, JMPR28, JMPR29, and JMPR31. As much 3 isolates were able to produce IAA and Gibberellin GA3 and 2 isolates were successfully analyzed by BLAST which showed the level of similarity to the species Bacillus Toyonensis and Bacillus Cereus. The results of this research were then implemented in booklet form as a biology learning resource with bacterial material.